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Coronatine-insensitive protein 1 (COI1), Recombinant Protein
Cat: RP00947
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Coronatine-insensitive protein 1 (COI1) , partial
Product Gene Name
COI1 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MEDPDIKRCK LSCVATVDDV IEQVMTYITD PKDRDSASLV CRRWFKIDSE TREHVTMALC YTATPDRLSR RFPNLRSLKL KGKPRAAMFN LIPENWGGYV TPWVTEISNN LRQLKSVHFR RMIVSDLDLD RLAKARADDL ETLKLDKCSG FTTDGLLSIV THCRKIKTLL MEESSFSEKD GKWLHELAQH NTSLEVLNFY MTEFAKISPK DLET
Sequence Positions
1-214. partial
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
67,665 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Coronatine-insensitive protein
NCBI/Uniprot Data
NCBI Accession #
NP_565919.1
NCBI GI #
18405209
NCBI GenBank Nucleotide #
NM_129552.4
NCBI GeneID
818581
NCBI Official Full Name
RNI-like superfamily protein
NCBI Official Symbol
COI1
NCBI Official Synonym Symbols
CORONATINE INSENSITIVE 1; T28M21.10; T28M21_10
NCBI Protein Information
RNI-like superfamily protein
NCBI Summary
Encodes a protein containing Leu-rich repeats and a degenerate F-box motif. Associates with AtCUL1, AtRbx1, and the Skp1-like proteins ASK1 and ASK2 to assemble SCF COI1 ubiquitin-ligase complexes in planta. A single amino acid substitution in the F-box motif of COI1 abolishes the formation of the SCF(COI1) complexes and results in loss of the JA response. Required for wound- and jasmonates-induced transcriptional regulation.
UniProt Gene Name
COI1
UniProt Synonym Gene Names
FBL2; AtCOI1; AtFBL2
UniProt Protein Name
Coronatine-insensitive protein 1
UniProt Synonym Protein Names
COI-1; F-box/LRR-repeat protein 2; AtCOI1; AtFBL2
UniProt Primary Accession #
O04197
UniProt Secondary Accession #
B2BD84
UniProt Comments
Required for jasmonate-regulated plant fertility and defense processes, and for coronatine and/or other elicitors perceptions/responses. Seems to not be required for meiosis. Required for the regulation of some genes induced by wounding, but not for all. Component of SCF(COI1) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins (probably including the ribulose bisphosphate carboxylase small chain 1B RBCS-1B and the histone deacetylase HDA6). These SCF complexes play crucial roles in regulating response to jasmonate, and their interactions with the COP9 signalosome (CSN) appear to be important for their activity. Interacts with TIFY10A and inositol pentakisphosphate to form a high-affinity jasmonates coreceptor. Involved in the regulation of plant gene expression during plant-pathogen interactions with Pseudomonas syringae and Alternaria brassicicola.
For research use only, not for clinical use.