CO | Constans (chicken antibody)

Inquiry

Cat: PA01165

Size: 50 µg

Host: Chicken

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana (recombinant CO)

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana CO protein, UniProt: Q39057, TAIR: At5g15840

Host

Chicken

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

for reconstitution please add 50 µl of dest, water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1: 1000 (WB)

Expected | apparent MW

41,9 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana (recombinant CO)

Predicted reactivity

Camelina sativa, Chrysanthemum sp., Solanum lycopersicum

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Recommended protocol for CO protein extraction Serrano-Bueno et al. 2020. Detection has to be done on a nuclear extract preparation in the evening on a Long Day that is the moment when CO protein is more stable.

Description

CO (CONSTANS) is a transcription factor thought to be involved in the long-day flowering pathway and is thought to have a regulatory role between the biological clock and flowering. It regulates flowering time through interactions with SOC1 (CO-1 inhibitor), TF1(top flower 1) and FT(flowering site T), and is involved in the biosynthesis of proline and ethylene.

For research use only, not for clinical use.