Clathrin heavy-chain 1,2
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Clathrin heavy-chain 1,2

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Cat: PA01646
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Actinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacumAS07 260
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)
Expected | apparent MW
193 | 170 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity
Actinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacumAS07 260
Predicted reactivity
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Populus trichocarpa, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays.
Not reactive in
Nicotiana benthamiana

Additional information

Please, note that fresh samples will provide better restuls (see image below)

Description

Clathrin is a protein involved in intracellular transport and plays an important role in the formation of coated vesicles. It consists of 3 clathrin heavy chains and 3 light chains. Clathrin-coated vesicles (CCVS) selectively sort cargo for multiple membrane transport routes in cell membranes, trans-Golgi networks, and endosomal compartments.

For research use only, not for clinical use.