Clathrin heavy-chain 1,2, ALP-conjugated (40 µg)
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Clathrin heavy-chain 1,2, ALP-conjugated (40 µg)

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Cat: PA00969
Size: 40 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Format
Liquid
Storage
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Application
Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)
Expected | apparent MW
193 | 170 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum
Predicted reactivity
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera
Not reactive in
Nicotiana benthamiana

Additional information

Please, note that fresh samples will provide better restuls (see image below)

Description

Clathrin is a protein involved in intracellular transport and plays an important role in the formation of coated vesicles. It consists of 3 clathrin heavy chains and 3 light chains. Clathrin-coated vesicles (CCVS) selectively sort cargo for multiple membrane transport routes in cell membranes, trans-Golgi networks, and endosomal compartments.

For research use only, not for clinical use.