Chlorpyrifos (polyclonal antibody)

AS13 2733 | Alkylphenols (nonylphenol; octylphenol), rabbit antibodies Antibodies to other toxic compounds,ELISA titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested in this technique. Plates are coated with 400 ng/ml ovalbumin conjugated-chlorpyrifos. Detection antibody is: anti-rabbit IgG, HRP-conjugated antibody in dilution 1: 8000. The titer in the indirect ELISA using OVA-conjugate is 500, in the indirect ELISA it is 100 000. The antibody is highly specific for chlorpyriphos showing no cross-reactivity with related and unrelated compounds. In the direct ELISA the detection limit is 1-4 nM (0.3-1.3 µg/L). Affinity and avidity are not determined. Specific antibody concentration is not determined as it is provided as a total immunoglobulin pool (purified by caprilic acid). Caprilic acid purified immunoglobulins can be used for most procedures like: coating plates for antigen capture assays and preparation of immunoaffinity columns. However, such preparations are not suitable for: conjugation with radioisotopes, enzymes, and biotin where contaminating proteins will reduce the specific activity..

Chlorpyrifos is a pesticide belonging to the organophosphorus sulfate group. It is used in vegetable cultivation and citrus orchards. Chlorpyrifos is an acetylcholinesterase inhibitor and therefore has a highly toxic effect on the nervous system. There is evidence that chlorpyrifos has an effect on the fetus and may cause behavioral disorders. Chlorpyrifos, Chinese Academy of Sciences No. : Solubility: methanol, isoctyl alcohol, benzene.