Cat | Catalase (peroxisomal marker)

Cat: PA00698

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Aponogeton madagascariensis, Brassica napus, Brassica oleracea, Fragaria x ananassa, Hordeum vulgare, Lathyrus sativus, Lupinus albus, Lupinus luteus, Moniliophthora perniciosa, Musa acuminate, Musa paradisiaca L., Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Paulownia tomentosa, Picrorhiza kurroa, Pisum sativum, Plumbago zeylanica, Setaria italica L. P. Beauv, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera Fragaria x ananassa

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide chosen from know plant catalase sequences including Arabidopsis thaliana isoforms: catalase-1 (Q96528, At1g20630), catalase-2 (P25819, At4g35090), catalase-3 (Q42547, At1g20620);

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunogold (IG), Immunolocalization (IL), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution

1: 500 (IG), 1: 25 - 1: 500 (IL), 2 µg (IP), 1 : 1000 (WB)

Expected | apparent MW

57 | 55 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Aponogeton madagascariensis, Brassica napus, Brassica oleracea, Fragaria x ananassa, Hordeum vulgare, Lathyrus sativus, Lupinus albus, Lupinus luteus, Moniliophthora perniciosa, Musa acuminate, Musa paradisiaca L., Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Paulownia tomentosa, Picrorhiza kurroa, Pisum sativum, Plumbago zeylanica, Setaria italica L. P. Beauv, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera Fragaria x ananassa

Predicted reactivity

Avicennia marina, Betula pendula, Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa subsp. pekinensis, Citrus sp., Citrus clementina, Citrus maxima, Camellia sinensis, Cucumis sativus, Cucurbita maxima, Cucurbita moschata, Elaeis guineensis var. tenera, Eucalyptus grandis, Glycine max, Gossypium mexicanum, Helianthus annus, Hibiscus cannabinus, Litchi chinensis, Lupinus albus, Manihot esculenta, Marchantia polymorpha, Morus notabilis, Nicotiana tabacum, Paenibacillus sp., Pinus pinea, Populus albaxtremula, Populus jackii, Prunus persica, Raphanus sativus, Saccharum officinarum, Sesamum indicum, Vicia faba

Not reactive in

Chlamydomonas reinhardtii

Additional information

This antibody is recognizing all three isoforms of Arabidopsis thaliana catalase. Catalase-2 is a main isoform expressed in leaf tissue and localized to peroxisomes. This antibody contains 0.1 % ProClin.,To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.To decrease background signal this antibody needs to be incubated in PBS-T NOT TBS-T. For reference, check image in application example below.

Description

Catalase is an enzyme present in most living organisms that catalyzes the breakdown of hydrogen peroxide into water and oxygen. In plant cells, catalase is present in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.

For research use only, not for clinical use.