Cas9 | CRISPR-associated endonuclease 9 (polyclonal)
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Cas9 | CRISPR-associated endonuclease 9 (polyclonal)

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Cat: PA00024
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Staphylococcus aureus
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunolocalization (IL), Western blot (WB)
Recommended dilution
1 : 100 (IL), 1 : 1000 (WB)
Expected | apparent MW
Depends upon a MW of a protein which is a fusion partner,

Reactivity

Confirmed reactivity
Staphylococcus aureus
Predicted reactivity
Staphylococcus pyogenes UniProt: Q99ZW2

Description

Cas9 (CRISPR-Associated endonucase 9) is an RNA-directed DNA endonucase associated with the clustered regularly interspersed short palindromic repeat (CRISPR) type II adaptive immune system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein is used as a genomic engineering tool to induce site-directed double-strand breaks in DNA.

For research use only, not for clinical use.