Lately localization to flagella has been confirmed for CAH6 Mackinder et al. 2017.Extraction method – harvest the cells and add SDS sample buffer.
CAH6(carbonic anhydrase 6) is a zinc-containing metalase that catalyzes the reversible hydration of carbon dioxide. There are three evolutionarily unrelated families of carbonic anhydrases: alpha, beta, and gamma. CAH6 belongs to the β family and is originally localized in chloroplast Mitra et al. (2004). However, the most recent location has been confirmed as flagellum Mackinder et al. 2017.
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