Shipping
The extracted protein is provided in vials at a concentration of 5mg/ml and shipped on dry ice. Protein can also be provided in denatured SDS Sample buffer for only Western blotting use.
Additional information
Quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands is verified by denatured SDS-PAGE using Coomassie blue staining. The integrity of protein is tested by immunoblotting using specific beta-actin antibody.
Application
The total protein can be immediately used for Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gel and SDS capillary electrophoresis, enzyme activity analysis, gel transfer test, protein protein interaction and tissue specific expression.
Description
Cabbage total protein (protein lysate) is isolated from freshly harvested tissues of single healthy normal donor. Tissues are homogenized in protein lysis buffer supplemented with a cocktail of protease inhibitors to minimize proteolysis and precisely quantified.