BRI1 | Brassinosteroid insensitive 1

This product can be sold containing proclin if requested,Antibody is tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans.   For IP: 15 µl GFP-trap beads is used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.Protein extraction has to be done efficiently as this step is crucial, recommended material to buffer ratio: 15 µl/µg or less.

BRI1 (Protein brassinosteroids INSENSITIVE 1) is a receptor that binds to brassinolide, has bispecial kinase activity, and acts on substrates containing serine/threonine and tyrosine. Involved in signaling cascades, including light and stress regulation of gene expression, promotes cell elongation, normal leaf and chloroplast aging and flowering. Alternative names :BRI1, brassinosteroid insensitive 1,CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR insensitive 1,ATBRI1, brassinosteroid LRR receptor kinase