BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1), partial

Product Gene Name

BAK1 recombinant protein

Product Synonym Gene Name

BAK1

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

NAEGD ALSALKNSLA DPNKVLQSWD ATLVTPCTWF HVTCNSDNSV TRVDLGNANL SGQLVMQLGQ LPNLQYLELY SNNITGTIPE QLGNLTELVS LDLYLNNLSG PIPSTLGRLK KLRFLRLNNN SLSGEIPRSL TAVLTLQVLD LSNNPLTGDI PVNGSFSLFT PISFANTKLT PLPASPPPPI SPTPPSPAGS NRITG

Sequence Positions

26-225

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

68,161 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Bcl-2 homologous antagonist/killer

NCBI Accession #

NP_567920.1

NCBI GI #

18418211

NCBI GenBank Nucleotide #

NM_119497.5

NCBI GeneID

829480

NCBI Official Full Name

BRI1-associated receptor kinase

NCBI Official Symbol

BAK1

NCBI Official Synonym Symbols

ATBAK1; ATSERK3; BRI1-associated receptor kinase; ELG; ELONGATED; F17M5.190; F17M5_190; RECEPTOR KINASES LIKE SERK 10; RKS10; SERK3; SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3

NCBI Protein Information

BRI1-associated receptor kinase

NCBI Summary

Leu-rich receptor Serine/threonine protein kinase. Component of BR signaling that interacts with BRI1 in vitro and in vivo to form a heterodimer. Brassinolide-dependent association of BRI1 and BAK1 in vivo. Phosphorylation of both BRI1 and BAK1 on Thr residues was BR dependent. Although BAK1 and BRI1 alone localize in the plasma membrane, when BAK1 and BRI1 are coexpressed, the heterodimer BAK1/BRI1 they form is localized in the endosome.

UniProt Gene Name

BAK1

UniProt Synonym Gene Names

AtSERK3

UniProt Protein Name

BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1

UniProt Synonym Protein Names

Protein ELONGATED; Somatic embryogenesis receptor kinase 3

UniProt Primary Accession #

Q94F62

UniProt Secondary Accession #

Q9SZC0; C0LGS0

UniProt Related Accession #

Q94F62

UniProt Comments

Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Controls the expression of genes associated with innate immunity in the absence of pathogens or elicitors. Involved in brassinosteroid (BR) signal transduction. Phosphorylates BRI1. May be involved in changing the equilibrium between plasma membrane-located BRI1 homodimers and endocytosed BRI1-BAK1 heterodimers. Interaction with MSBP1 stimulates the endocytosis of BAK1 and suppresses brassinosteroid signaling. Acts in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) via its interactions with FLS2 and EFR, and the phosphorylation of BIK1. Involved in programmed cell death (PCD) control. Positively regulates the BR-dependent plant growth pathway and negatively regulates the BR-independent cell-death pathway (PubMed:17583510, PubMed:17600708, PubMed:18667726, PubMed:18694562, PubMed:19124768, PubMed:20018402, PubMed:20404519, PubMed:21350342, PubMed:21693696). Phosphorylates BIR2 and thus promotes interaction with BIR2 (PubMed:24388849, PubMed:24556575). This interaction prevents interaction with FLS2 in the absence of pathogen-associated molecular patterns (PAMP) (PubMed:24388849, PubMed:24556575). Required for PSK promotion of seedling growth and protoplast expansion (PubMed:26071421). CNGC17 and AHAs form a functional cation-translocating unit that is activated by PSKR1/BAK1 and possibly other BAK1/RLK complexes (PubMed:26071421).