BirA (mutated/TurboID) - DyLight 650 conjugated (40 µg)
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BirA (mutated/TurboID) - DyLight 650 conjugated (40 µg)

Cat: PA00143
Size: 40 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: BirA (mutated/TurboID)
Datasheet:

Product Info

Immunogen
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression is done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Host
Rabbit
Clonality
Polyclonal
Purity
Antigen affinity purified serum
Format
Liquid in PBS pH 7,4.
Storage
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
Application
Immunofluorescence (IF), Western blot (WB)
Recommended dilution
To be determined by end user.
Expected | apparent MW
Depends upon fusion partner

Reactivity

Confirmed reactivity
BirA (mutated/TurboID)
Not reactive in
No confirmed exceptions from predicted reactivity are currently known,

Description

Using TurboID to label proteins allows the study of protein interactions in different types of cells and organs, as well as developmental stages, and is a suitable tool for approaching labeling experiments as described in maair et al. (2019). TurboID Supports proximity labeling of Arabidopsis protein Complexes and cell type-specific organelles proteome, Elife, September 19, 2019; 8:e47864, doi: 10,7554/ Elife,47864, the MW of this label is 35 kDa. DyLight 650 Amax = 652 nm, Emax = 672 nm. DyLight is a registered trademark of Thermofisher Inc. and its subsidiaries.

For research use only, not for clinical use.