BiP | Lumenal-binding protein (rabbit antibody)
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BiP | Lumenal-binding protein (rabbit antibody)

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Cat: PA00411
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Anacardium occidentale, Arabidopsis thaliana, Brassica napus,Chara australis R.Br, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Oryza sativa, Picea abies, Pistachio sp., Physcomitrium patens, Schinus molle, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zea mays
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540  Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080  Q8H1B3
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
ELISA (ELISA), Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
Recommended dilution
1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)
Expected | apparent MW
73,5 | 80 kDa

Reactivity

Confirmed reactivity
Anacardium occidentale, Arabidopsis thaliana, Brassica napus,Chara australis R.Br, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Oryza sativa, Picea abies, Pistachio sp., Physcomitrium patens, Schinus molle, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zea mays
Predicted reactivity
Arabis alpina, Capsella rubella, Capsicum annuum, Citrus clementina, Citrus sinsensis, Eucalyptus grandis, Glycine max, Hordeum vulgare, Isatis tincorina, Prunus persica, Triticum aestivium, Petunia hybrida, Picea sitcHensis, Populus trichocarpa, Ricinus comminus, Vitis vinifera
Not reactive in
Ostreococcus tauri

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel, This antibody has so far not worked in IP

Description

BiP2 (Binding immunoglobulin protein, binding immunoglobulin protein) is located in endoplasmic reticulum lumen (ER) and participates in protein assembly in the ER. BiP proteins are abundant under all growth conditions, but their synthesis increases under conditions of accumulation of unfolded peptides in the endoplasmic reticulum (ER).

For research use only, not for clinical use.