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BiP | Lumenal-binding protein (goat antibody)
Cat: PA00790
Size: 100 µg
Host: Goat
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Spinacia oleracea, Zea mays
Datasheet:
Product Info
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 UniProt:Q9LKR3, TAIR: At5g28540, BiP2 UniProt: F4K007, TAIR: At5g42020, BiP3 UniProt:Q8H1B3 ,TAIR:At1g09080
Host
Goat
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
73,5 | 80 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Hordeum vulgare, Spinacia oleracea, Zea mays
Predicted reactivity
Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Populus trichocarpa, Physcomitrium patens, Spinacia oleracea, Zea mays
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Additional information
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. Antibody has a reduced reactivity to monocots in western blot.
Description
BiP2 (Binding immunoglobulin protein, binding immunoglobulin protein) is located in endoplasmic reticulum lumen (ER) and participates in protein assembly in the ER. BiP proteins are abundant under all growth conditions, but their synthesis increases under conditions of accumulation of unfolded peptides in the endoplasmic reticulum (ER). Alternative name :AtBP2
For research use only, not for clinical use.