BetaCA1 | Beta carbonic anhydrase 1 (chloroplastic)

Inquiry

Cat: PA00913

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide, derived in the part C-terminus of BetaCA1 of Arabidopsis thaliana, UniProt: P27140, TAIR: At3g01500

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 20 000 (WB)

Expected | apparent MW

37,5 | 25,3 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Solanum lycopersicum Q5NE20

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Extraction method – Grind 50 mg of leaf tissue in a sterile microcentrifuge tube using a sterile plastic pestle. Add 132μL of Protein Extraction Buffer (1x TE, 1.2 %SDS, 2.7% sucrose, 7.5 μg mL-1 bromophenol blue) to the ground leaf tissue. Vortex the sample and keep on ice for 15 mins. Centrifuge at 14,000 rpm for five minutes using a benchtop centrifuge. Collect the supernatant and in a new sterile 0.5 ml microcentrifuge tube and discard the pellet.This antibody does not recognize betaCA2.

Description

Beta-carbonic anhydrase 1 (beta-carbonic anhydrase 1)(chloroplast)- is a zinc metalloenzyme that converts CO2 and HCO3(-) to each other. Aliases: Arabidopsis salicylic acid-binding protein 3,ATBCA1, ATSABP3, carbonic anhydrase 1,CA1, carbonic anhydrase 1,SABP3, salicylic acid-binding protein 3.

For research use only, not for clinical use.