Shipping
Extracted proteins are vialed at 5 mg/mL and shipped on dry ice. The protein is also available in Denaturing SDS Sample Buffer for Western blotting only.
Additional information
The clarity and resolution of protein bands were validated using denatured SDS-PAGE and Coomassie blue staining. Using specificity β - Immunoblotting with actin antibody was used to detect the integrity of the protein.
Application
Total protein is ready for immediate use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, protein-protein interaction, and tissue specific expression.
Description
Barley total protein (protein lysate) is isolated from freshly harvested tissues of single healthy normal donor. Tissues are homogenized in protein lysis buffer supplemented with a cocktail of protease inhibitors to minimize proteolysis and precisely quantified.