BAK1 | Brassinosteroid insensitive 1-associated receptor kinase 1
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BAK1 | Brassinosteroid insensitive 1-associated receptor kinase 1

Cat: PA00912
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Solanum lycopersicum
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide, chosen from Arabidopsis thaliana Bak1 sequence, TAIR: AT4G33430 UniProt: Q94F62
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
2 µl/50 µl of Protein G agarose, 1 : 5000 (WB)
Expected | apparent MW
68 | 70 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Solanum lycopersicum
Predicted reactivity
Thelungiella halophila
Not reactive in
Hordeum vulgare, Nicotiana benthamiana, Oryza sativa

Additional information

Antibodies bind to AtBAK1-Myc. They do not cross react with AtSOBIR.This product can be sold containing proclin if requested.,Extra Information on CE extraction buffer: CE buffer does not need to be made freshly everytime. Aliquots can be kept at -20°C. Na2MoO4 and NaF are phosphatase inhibitors, included to prevent lose phosphorylation form our protein of interest during extraction. EDTA chelates metal ions and thus inhibits many enzymes which need metal ions as co-factors and inhibits the action of proteases.  Protease inhibitor coctail is Sigma product number P 9599 which is used in dilution 1: 100.Antibody is reconizing AtBAK1-Myc in total extracts.

Description

In the absence of pathogens or elicitors, BAK1(brassinolide Insensitive 1 associated receptor kinase 1) controls the expression of genes associated with innate immunity. Phosphorylated Brl1. Involved in programmed cell death (PCD). Subcellular location: Cell membrane. Alternative names: brassinosteroid insensitive 1 associated receptor kinase 1,BAK1, BRI1-associated receptor kinase 1, RKS10, receptor kinase-like SERK 10, SERK3, Somatic embryogenic receptor-like kinase 3,ELG, Elongate, ATSERK3, somatic embryogenic receptor-like kinase 3,ATBAK1.

For research use only, not for clinical use.