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Auxin response factor 1 (ARF1), Recombinant Protein
Cat: RP01926
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Auxin response factor 1 (ARF1) , partial
Product Gene Name
ARF1 recombinant protein
Product Synonym Gene Name
ARF1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
73,370 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Auxin response factor
NCBI/Uniprot Data
NCBI Accession #
NP_001031208.1
NCBI GI #
79320256
NCBI GenBank Nucleotide #
NM_001036131.3
NCBI GeneID
842268
NCBI Official Full Name
auxin response factor 1
NCBI Official Symbol
ARF1
NCBI Official Synonym Symbols
auxin response factor 1; F23H11.7; F23H11_7
NCBI Protein Information
auxin response factor 1
NCBI Summary
Encodes a member of the auxin response factor family. ARFs bind to the cis element 5'-TGTCTC-3' ARFs mediate changes in gene expression in response to auxin. ARF's form heterodimers with IAA/AUX genes. ARF1 enhances mutant phenotypes of ARF2 and may act with ARF2 to control aspects of maturation and senescence.ARF1:LUC and 3xHA:ARF1 proteins have a half-life of ~3-4 hours and their degradation is reduced by proteasome inhibitors. 3xHA:ARF1 degradation is not affected by a pre-treatment with IAA. A nuclear-targeted fusion protein containing the middle region of ARF1 linked to LUC:NLS has a similar half-life to the full-length ARF1:LUC construct. The degradation of 3xHA:ARF1 is not affected in an axr6-3 mutant grown at room temperature, although the degradation of AXR2/IAA7 is slowed under these conditions.
UniProt Gene Name
ARF1
UniProt Protein Name
Auxin response factor 1
UniProt Primary Accession #
Q8L7G0
UniProt Secondary Accession #
O23664
UniProt Related Accession #
Q8L7G0
UniProt Comments
Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Promotes flowering, stamen development, floral organ abscission and fruit dehiscence. Acts as repressor of IAA2, IAA3 and IAA7.
For research use only, not for clinical use.