AtpH | ATP synthase subunit c (chloroplastic)

Inquiry

Cat: PA00497

Size: 200 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus

Datasheet:

Product Info

Immunogen

Purified c subunit from Spinacia oleracea UniProt: P69447.

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000-1 : 10 000 (WB)

Expected | apparent MW

8 kDa (for Arabidopsis thaliana)

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus

Predicted reactivity

Algae, Cannabis sativa, Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus alba, Pinus thunbergii, Zea mays, Vitis vinifera

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

This product can be sold containing proclin if requested.,Note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e,g, 5 or 6 subunits) may occur

Description

F-type ATP synthase (ATP synthase) is a general-purpose enzyme that synthesizes ATP from ADP and phosphate using energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build a ring structure that binds to the O-part membrane of the enzyme (14-mer in spinach, about 112 kDa).

For research use only, not for clinical use.