ATP-dependent DNA helicase DDM1 (DDM1), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana ATP-dependent DNA helicase DDM1 (DDM1) , partial

Product Gene Name

DDM1 recombinant protein

Product Synonym Gene Name

DDM1

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

86,638 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

ATP-dependent DNA helicase

NCBI Accession #

NP_201476.1

NCBI GI #

15240074

NCBI GenBank Nucleotide #

NM_126073.3

NCBI GeneID

836808

NCBI Official Full Name

chromatin remodeling 1

NCBI Official Symbol

CHR1

NCBI Official Synonym Symbols

ATDDM1; CHA1; CHR01; chromatin remodeling 1; CHROMATIN REMODELING 1; DDM1; DECREASED DNA METHYLATION 1; MSN2.14; MSN2_14; SOM1; SOM4; SOMNIFEROUS 1

NCBI Protein Information

chromatin remodeling 1

NCBI Summary

Protein is similar to SWI2/SNF2 chromatin remodeling proteins. DDM1 is appears to act as a chromatin-remodeling ATPase involved in cytosine methylation in CG and non-CG contexts. Involved in gene silencing and maintenance of DNA methylation and histone methylation. Hypomethylation of many genomic regions occurs in ddm1 mutants, and can cause several phenotypic abnormalities, but some loci, such as BONSAI (At1g73177) can be hypermethylated in ddm1 mutants after several generations, leading to different phenotypes. DDM1 might be involved in establishing a heterochromain boundary. A line expressing an RNAi targeted against DDM1 shows some resistance to agrobacterium-mediated root transformation.

UniProt Gene Name

DDM1

UniProt Synonym Gene Names

; AtCHR1; CHR01; AtDDM1

UniProt Protein Name

ATP-dependent DNA helicase DDM1

UniProt Synonym Protein Names

Protein CHROMATIN REMODELING 1

UniProt Primary Accession #

Q9XFH4

UniProt Secondary Accession #

Q5C995; Q5C996

UniProt Related Accession #

Q9XFH4

UniProt Comments

ATP-dependent DNA helicase that plays a role in formation, organization, stability and heritability of heterochromatin and thus regulates several physiological traits. Binds to the nucleosome and promotes chromatin remodeling in an ATP-dependent manner; induces nucleosome repositioning on a short DNA fragment, and, possibly, could be guided to target sites (including silent transposable elements) by small interfering RNAs (siRNAs). Can bind both free and nucleosomal DNA. Required for the heritable maintenance of genome integrity and transcriptional gene silencing (TGS), including homology-dependent gene silencing (HDG silencing), via the maintenance of DNA methylation (mostly on cytosine, in both CpG and CpHpG sites, where H is A, T or C) and of histone methylation (e.g. chromatin methylation). May facilitate localization of MBD proteins at specific nuclear domains. Necessary for the maintenance of the genomic imprint at the MEA locus, especially for the silencing of paternally inherited MEA locus. Plays a major role in the inactivation maintenance of retrotransposons (e.g. Tar17, SINE, LINE, ATLN39, CAC1 (CACTAs), Athila elements, and mutator-like elements MULEs and TIR-MULEs) and the silencing of repeated genes and transgenes (e.g. T-DNA insertions). Required for KYP-dependent histone H3 'Lys-9' (H3K9me) methylation, deacetylation of histone H4 'Lys-16' (H4K16) and MET1-dependent DNA methylation. Involved in the chromatin organization of 5S rRNA genes (localized in the pericentromeric heterochromatin of chromosomes 3, 4, and 5) modifications during heterochromatin establishment. Prevents siRNA accumulation (siRNA are probably involved in epigenetic inheritance and in 5S rRNA genes regulation by silencing). Required during plant organogenesis and development, as well as during seed formation.