ATG7 | Autophagy-related protein 7

Inquiry

Cat: PA01219

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

Recombinant ATG7 of Arabidopsis thaliana, UniProt: Q94CD5-1, TAIR: At5g45900, overexpressed in E.coli, collected in inclusion bodies, which are solubilized before immunization

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 3000 (WB)

Expected | apparent MW

76,5 | 75 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Brassica rapa, Capsella rubella , Citrus clementina, Noccaea caerulescens

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Protein name is changed from APG7 to ATG7 after Doelling paper is published.

Description

Autophagy-related protein 7 (ATG7) is an E1-like activating enzyme involved in two ubiquitin-like systems required for cytoplasmic vacuolar transport (Cvt) and autophagy. Activate the binding of ATG12 and ATG5, and activate the binding of ATG8 and phosphatidylethanolamine. In the process of leaf yellowing induced by stress, it is involved in aging process, degradation of damaged peroxisome and non-selective degradation of chlorophyll and photosynthetic proteins. Aliases: ubiquitin-like modifier activating enzyme atg7, atg12 activating enzyme E1 atg7, AtAPG7, abnormal localization of protein peroxisome 4.

For research use only, not for clinical use.