Shipping
The extracted protein is provided in vials at a concentration of 5mg/ml and shipped on dry ice. Protein can also be provided in denatured SDS Sample buffer for only Western blotting use.
Additional information
Protein quality can be examined by denaturing SDS-PAGE analysis, protein band clarity and resolution using Coomassie Blue staining. Protein integrity was examined by immunoblotting using a specific beta-actin antibody.
Application
Total protein is ready for immediate use in Western blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis, enzymatic activity analysis, gel shifting assay, protein-protein interaction, and tissue specific expression.
Description
Asparagus total protein (protein lysate) is isolated from freshly harvested tissues of single healthy normal donor. Tissues are homogenized in protein lysis buffer supplemented with a cocktail of protease inhibitors to minimize proteolysis and precisely quantified.