ARF1 | ADP-ribosylation factor 1

Cat: PA00405

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Actinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Elaeis sp. , Lilium longiflorum, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Petunia hybrida cv Mitchell, Physcomitrium patens, Solanum tuberosum

Datasheet:

Product Info

Immunogen

Recombinant GST fusion of full length of Arabidopsis thaliana ARF1 (P36397, AT2G47170)

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunofluorescence (IF), Immunogold (IG), Western blot (WB)

Recommended dilution

1 : 1000 (IF), 1 : 100 (IG), 1 : 1000 (WB)

Expected | apparent MW

21 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity

Actinidia chinensis, Arabidopsis thaliana, Chlamydomonas reinhardtii, Elaeis sp. , Lilium longiflorum, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Petunia hybrida cv Mitchell, Physcomitrium patens, Solanum tuberosum

Predicted reactivity

Brassica juncea, Brassica napus, Capsella rubella, Capsicum annum, Cucumis sp., Daucus carota, Elaeis guineensis, Glycine max, Helleborus orientalis, Hordeum vulgare, Medicago truncatula, Nannochloropsis gaditana, Nicotiana benthamina, Ostreococcus tauri, Populus trichocarpa, Triticum aestivum, Zea mays

Not reactive in

Microsporidia sp.

Additional information

Cellular [compartment marker] of Golgi in immunolocalization and COP1 in western blot,References describing immunolocalization (IF) and (IG) studies: Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36. Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.

Description

ARF1 protein is located in the Golgi apparatus and plays a central role in Golgi transport in vivo. It is a small GTP enzyme that undergoes the GDP/GTP nucleotide exchange cycle and is an important regulator of cell transport.

For research use only, not for clinical use.