Arachis hypogaea Lectin (PNA) - Cy5
Description
Peanut lectin (PNA) is purified by affinity chromatography and consists of four subunits, each of about 27,000 subunits. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It is carbohydrate-specific for Galβ3GalNAc with galactose-eluting sugar. Galactose and lactose are poor inhibitors, while disaccharide galactose beta (1->3)GalNAc and defatalized human red blood cell substance N (t antigen, present in many M and N blood groups) are potent inhibitors of PNA. PNA can be used in clinical detection of red blood cell T-polyagglutination and detection of normal and malignant cell membranes. This lectin can be used to characterize tumor-specific antigens on the surface of malignant cells. It can be used in the isolation of mouse stem cells for bone marrow transplantation across histocompatibility barriers. After Cy5 binds to peanut lectin (PNA), the binding pattern of this lectin can be shown in cell imaging and flow cytometry. The excitation wavelength required for Cy5 fluorescence is high enough to avoid overlapping with most other fluorescent dyes, making it useful for double-labeling experiments. Because of this high excitation, the autofluorescent background of biological specimens is usually less. This product is a stable liquid.
Product Info
For research use only, not for clinical use.