Arachis hypogaea Lectin (PNA) - Cy3
Description
Peanut lectin (PNA) is purified by affinity chromatography and consists of four subunits, each of about 27,000 subunits. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It is carbohydrate-specific for Galβ3GalNAc with galactose-eluting sugar. Galactose and lactose are poor inhibitors, while disaccharide galactose beta (1->3)GalNAc and defatalized human red blood cell substance N (t antigen, present in many M and N blood groups) are potent inhibitors of PNA. PNA can be used in clinical detection of red blood cell T-polyagglutination and detection of normal and malignant cell membranes. This lectin can be used to characterize tumor-specific antigens on the surface of malignant cells. It can be used in the isolation of mouse stem cells for bone marrow transplantation across histocompatibility barriers. Affinity purified PNA binds to cyanine 3 (Cy3) fluorescent dye. Cyanine dyes are better able to withstand harsh dehydration and the embedding conditions required to install parts in non-polar plastic media, and are brighter in non-polar environments than in water-based media. The advantages of plastic over water-based mounting media are brightness, contrast and fluorescence lifetime. Cy3 conjugates have the advantage of bright light stability in both aqueous environments and non-polar media. This product is a stable liquid.
Product Info
For research use only, not for clinical use.