Arachis hypogaea Lectin (PNA) - Biotinylated
Description
Peanut lectin (PNA) is purified by affinity chromatography and consists of four subunits, each of about 27,000 subunits. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It is carbohydrate-specific for Galβ3GalNAc with galactose-eluting sugar. Galactose and lactose are poor inhibitors, while disaccharide galactose beta (1->3)GalNAc and defatalized human red blood cell substance N (t antigen, present in many M and N blood groups) are potent inhibitors of PNA. Biotin is a small molecule involved in a variety of metabolic processes. This ligand forms complexes with avidin and streptavidin, resulting in the strongest known non-covalent protein-ligand interaction. Biotinylated peanut lectin (PNA) has a moderate amount of biotin binding and provides the best detection characteristics when using avidin-HRP or streptavidin-HRP couplers. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.
Product Info
For research use only, not for clinical use.