AOX1/2 | Plant alternative oxidase 1 and 2

Cat: PA00191

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Betula nana, Beta vulgaris, Brassica napus, Brassica oleracea, Kandelia candel, Eriphorum vaginatum, Hordeum vulgare, Lupinus luteus, Nicotiana tabacum, Oryza sativa, Picea abies, Pisum sativum, Poa annua, Robinia pseudoacacia, Rosa hybrida, Solanum lycopersicum, Solanum tuberosum, Symplocarpus renifolius, Physcomitrium patens, Tigriopus californicus, Triticum aestivum, Zea mays

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms including Arabidopsis thaliana AOX1A. UniProt: Q39219, TAIR: At3g22370, AOX1B UniProt: O23913, TAIR:AT3G22360, AOX1C UniProt: O22048, TAIR: AT3G27620, and AOX2, UniProt: O22049, TAIR: AT5G64210, Solanum lycopersicum UniProt: Q7XBG9, Oryza sativa UniProt: Q7XT33, AOX1D, TAIR: AT1G32350

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunolocalization (IL), Western blot (WB)

Recommended dilution

1 : 750 (IL), 1 : 1000 for 10-20 µg of mitochondrial protein/lane detection (WB)

Expected | apparent MW

36-40 | 36-40 for Arabidopsis thaliana

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Betula nana, Beta vulgaris, Brassica napus, Brassica oleracea, Kandelia candel, Eriphorum vaginatum, Hordeum vulgare, Lupinus luteus, Nicotiana tabacum, Oryza sativa, Picea abies, Pisum sativum, Poa annua, Robinia pseudoacacia, Rosa hybrida, Solanum lycopersicum, Solanum tuberosum, Symplocarpus renifolius, Physcomitrium patens, Tigriopus californicus, Triticum aestivum, Zea mays

Predicted reactivity

Aegilops tauschii, Brachypodium distachyon, Capsella rubella, Citrus sinensis, Citrus clementina, Corylus heterophylla, Crocus sativus, Cucumis sativus, Daucus carota, Glycine max, Hypericum perforatum, Lotus japonicus, Malus x domestica, Medicago truncatula, Medicago sativa, Naegleria gruberi (amoeba), Nelumbo nucifera, Nicotiana benthamiana, Oryza brachyantha, Populus tremula, Picea sitchensis,Pyrus communis,Saccharum officinarum, Sauromatum venosum, Sorghum bicolor, Selaginella moellendorffii, Tetrahymena thermophila, Vigna radiata, Vigna unguiculata, Vitis vinifera

Not reactive in

Candidia albicans, Chlamydomonas reinhardtii (use an antibody to algal AOX1, AS06 152)

Additional information

Mitochondrion inner membrane marker. Possibly in the inner surface of the inner mitochondrial membrane.Protocol for a plant mitochondria preparation can be found here. In protein samples which are older than few months AOX enzyme can undergo intensive dimerization. Such preparations should not be used to work with this antibody.,According to Konert et al. (2015) AOX antibody is recognizing AOX1A and AOX1D.This product can be sold containing ProClin if requested.

Description

Replacement oxidase (AOX) is a quinoline oxidase located in the membrane of plant mitochondria. They act as terminal oxidases in alternating electron transport pathways, oxidizing ubiquinone to reduce oxygen to water.

For research use only, not for clinical use.