AOX | AOX positive control/quantitation standard

The AOX calibrated protein standard can be used in combination with Agrisera global anti-AOX antibodies to quantitate AOX from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence.  Quantitative western blot:  detailed method description, video tutorial,Concentration: 0.1 pmol/µl. After re-constitution with sterile milliQ water, the final concentration of the AOX monomer is.0.1 pmol/µl. While a dimer is present in the lane, only the 27 kDa monomer contributes to the calibration. Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.

Replacement oxidase (AOX) is a quinoline oxidase located in the membrane of plant mitochondria. They act as terminal oxidases in alternating electron transport pathways, oxidizing ubiquinone to reduce oxygen to water. AOX standard source: The AOX standard source is Sphingosphinomonas Wilsoni RW1, which is overexpressed in Escherichia coli and carries the n-terminal his6 label.