AO | ascorbate oxidase

Inquiry

Cat: PA00637

Size: 1 mg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Cucurbita sp.

Datasheet:

Product Info

Immunogen

ascorbate oxidase purified from Cucurbita sp.

Host

Rabbit

Clonality

Polyclonal

Purity

Total IgG in PBS pH 7.2.

Format

Lyophilized

Reconstitution

For reconstitution add 0,5 ml of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.

Application

ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution

1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)

Expected | apparent MW

61 | 45 kDa (Cucurbita pepo)

Reactivity

Confirmed reactivity

Cucurbita sp.

Predicted reactivity

Glycne max, Oryza sativa

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography,

Description

Ascorbate oxidase (AO) is an extracellular enzyme involved in plant ascorbic acid (AA) metabolism. Ascorbic acid (AA) plays a key role in the resistance to oxidative stress and is especially abundant in photosynthetic tissues. More than 90% of ascorbate is localized in the cytoplasm, but a considerable proportion is exported to the exosomes.

For research use only, not for clinical use.