AO | ascorbate oxidase, Biotin conjugated
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AO | ascorbate oxidase, Biotin conjugated

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Cat: PA00638
Size: 10 mg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Cucurbita sp.
Datasheet:

Product Info

Immunogen
ascorbate oxidase purified from Cucurbita sp.
Host
Rabbit
Clonality
Polyclonal
Purity
Purified IgG in PBS pH 7.2.
Format
Lyophilized
Reconstitution
For reconstitution add 1 ml of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.
Application
ELISA (ELISA), Western blot (WB)
Recommended dilution
1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW
61 | 45 kDa (Cucurbita pepo)

Reactivity

Confirmed reactivity
Cucurbita sp.
Predicted reactivity
Glycne max, Oryza sativa
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography, N-Hydroxysuccinimidobiotin is used for labelling of antibody,IgG concentration is 10 mg/ml, Biotin/IgG protein molar ratio is approximately 5,6, No foreign proteins are added

Description

Ascorbate oxidase (AO) is an extracellular enzyme involved in plant ascorbic acid (AA) metabolism. Ascorbic acid (AA) plays a key role in the resistance to oxidative stress and is especially abundant in photosynthetic tissues. More than 90% of ascorbate is localized in the cytoplasm, but a considerable proportion is exported to the exosomes.

For research use only, not for clinical use.