Amidase 1 (AMI1), Recombinant Protein
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Amidase 1 (AMI1), Recombinant Protein

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Cat: RP01496
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Amidase 1 (AMI1)
Product Gene Name
AMI1 recombinant protein
Product Synonym Gene Name
AMI1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MATNNDFGAF IEKVTISPTS TSSSPPSLQG LTFAIKDIFD VEGRVTGFGN PDWLRTHSAA TSTAPVVSSL LEAGATALGI TIMDEMAYSI NGENAHYGTP RNPIAFDRVP GGSSSGSAVA VAARLVDFSI GTDTGGSVRV PASYCGIFGF RPSHGAVSTV GLTPMAQSFD TVGWFARDTA TLKRVGCVLL QQHHLNPIEP SQLIIADDCF KLCSVPHDLL VQPLVGSVEK SFGGNTVVKK VNLGEYIGQN VPSLKHFMTS DDVTTQQEFC IPSLMALSSS MRLLQRHEFK INHGAWISSV KPEFGPGISE RIEEAIRTSD EKIDHCRSVK SELITALSTL LGEKGVLVIP TVPGPPPHLQ ANVAALESFR SRAFSLLSIA GVSGFCQVSI PLGLHENLPV SVSLVAKYGS DGFLLSLVDS LAAFI
Sequence Positions
1-425, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
45,056 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Amidase

NCBI/Uniprot Data

NCBI Accession #
NP_563831.1
NCBI GI #
18390964
NCBI GenBank Nucleotide #
NM_100769.4
NCBI GeneID
837418
NCBI Official Full Name
amidase 1
NCBI Official Symbol
AMI1
NCBI Official Synonym Symbols
amidase 1; AMIDASE-LIKE PROTEIN 1; ARABIDOPSIS THALIANA TRANSLOCON AT THE OUTER MEMBRANE OF CHLOROPLASTS 64-I; ATAMI1; ATTOC64-I; TOC64-I; TRANSLOCON AT THE OUTER MEMBRANE OF CHLOROPLASTS 64-I
NCBI Protein Information
amidase 1
NCBI Summary
Encodes an enzyme with similarity to bacterial acylamidohydrolases and exhibits indole-3-acetamide amidohydrolase activity in vitro. This enzyme may be involved in the in vivo biosynthesis of indole-acetic acid from indole-3-acetamide, a native metabolite of A. thaliana. It appears to exist as a monomer.
UniProt Gene Name
AMI1
UniProt Synonym Gene Names
AtTOC64-I
UniProt Protein Name
Amidase 1
UniProt Synonym Protein Names
Translocon at the outer membrane of chloroplasts 64-I
UniProt Primary Accession #
Q9FR37
UniProt Secondary Accession #
O04032
UniProt Related Accession #
Q9FR37
UniProt Comments
Amidase involved in auxin biosynthesis. Converts indole-3-acetamide to indole-3-acetate (PubMed:12620340, PubMed:27135507). Converts phenyl-2-acetamide (PAM) to phenyl-2-acetate. Substrate preference is PAM > IAM (PubMed:27135507). Can also use L-asparagine, oleamide and 1-naphtalene-acetamide as substrates, but not indole-3-acetonitrile or indole-3-acetyl-L-aspartic acid (PubMed:12620340).

For research use only, not for clinical use.