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Alcohol Dehydrogenase 1 (ADH1), Recombinant Protein
Cat: RP01059
Size: 0.01 mg/ 0.05 mg/ 0.1 mg/ 0.2 mg/ 0.5 mg/ 1 mg/ 5 mg/ 5x5 mg
Species: Arabidopsis halleri
Datasheet:
Product Info
Full Product Name
Recombinant Alcohol Dehydrogenase 1 (ADH1)
Product Gene Name
ADH1 recombinant protein
Purity
> 95%
Sequence
Residues: Lys169~Arg 370 (Accession # P07327), with two N-terminal Tags, His-tag and T7-tag. The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG LGGVGLSAIM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPDSQ NLSMNPMLLL TGRTWKGAIL GGFKSKECVP KLVADFMAKK FSLDALITHV LPFEKINEGF DLLHSGKSIR
Chromosome Location
Chromosome: 1; NC_003070.9 (28975394..28977417). Location: chromosome: 1
Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Host
E Coli
Molecular Weight
25.1kDa
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8℃ for one month. Aliquot and store at -80℃ for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37℃ for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Protein Family
Alcohol dehydrogenase
NCBI/Uniprot Data
NCBI Accession #
NP_177837.1
NCBI GI #
15223838
NCBI GenBank Nucleotide #
NM_106362.2
NCBI GeneID
844047
NCBI Official Full Name
alcohol dehydrogenase 1
NCBI Official Symbol
ADH1
NCBI Official Synonym Symbols
ADH; ADH1; ALCOHOL DEHYDROGENASE; alcohol dehydrogenase 1; ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE; ATADH; ATADH1; F22K20.19; F22K20_19
NCBI Protein Information
alcohol dehydrogenase 1
NCBI Summary
Catalyzes the reduction of acetaldehyde using NADH as reductant. Requires zinc for activity. Dimer. Anaerobic response polypeptide (ANP). Fermentation. The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide.
UniProt Gene Name
ADH1
UniProt Synonym Gene Names
ADH
UniProt Protein Name
Alcohol dehydrogenase class-P
UniProt Entry Name
ADH1_ARATH
UniProt Primary Accession #
P06525
UniProt Secondary Accession #
O04080; O04713; O04717; O04868; O23821; Q8LA61; Q94AY6; Q9CAZ2; Q9CAZ3
UniProt Related Accession #
P06525
UniProt Comments
Catalytic activity: An alcohol + NAD+ = an aldehyde or ketone + NADH.Cofactor: Binds 2 zinc ions per subunit.Subunit structure: Homodimer.Subcellular location: Cytoplasm. Induction: By 2,4-dichlorophenoxyacetic acid in Arabidopsis as well as in maize.Miscellaneous: Unlike most plants, Arabidopsis contains only one gene for ADH.Sequence similarities: Belongs to the zinc-containing alcohol dehydrogenase family. Class-P subfamily.
For research use only, not for clinical use.