For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1,In the work of Honsbein et al, 125I has been used for detection of KC1 since this is the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)
AKT1 is a highly selective potassium channel located within the cell membrane that mediates the uptake of potassium by plant roots under low potassium conditions.
For research use only, not for clinical use.