AKT1 | Potassium channel AKT1
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AKT1 | Potassium channel AKT1

Cat: PA01174
Size: 200 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 50 with 125I (WB)
Expected | apparent MW
96,9 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1,In the work of Honsbein et al, 125I has been used for detection of KC1 since this is the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)

Description

AKT1 is a highly selective potassium channel located within the cell membrane that mediates the uptake of potassium by plant roots under low potassium conditions.

For research use only, not for clinical use.