AGO9 | Argonaute 9
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AGO9 | Argonaute 9

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Cat: PA01337
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO9 protein sequence UniProt: Q84YI4, TAIR:At5g21150.
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunolocalization, whole-mount (IL), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
5 µg of antibody per 1 gram of a fresh tissue (IP), 1 : 10 000 (WB)
Expected | apparent MW
101 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.A recommended whole-mount immunolocalization protocol can be found here.

Description

AGO9 belongs to a group of argonaute proteins that are catalytic components of the RNA-including Silencing complex (RISC).

For research use only, not for clinical use.