AGO6 | Argonaute 6

Inquiry

Cat: PA01336

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

116,4 | 99 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Arabidopsis thaliana

Not reactive in

Zea mays

Additional information

There can be a reaction with NEB broad range prestained protein ladder depending upon secondary antibody used. AGO6 accumulates to lower levels in the nrpd1a mutant (Havecker at el. 2010). In addition, a non-specific band migrates to a similar location and thus protein is run on a 6% denaturing gel to obtain good separation. If other secondary antibody is used than in the figure below, cross-reacting pattern can be different.,AGO6 expression is cell specific, Seedlings can be used as a negative control

Description

AGO6 (Argonaute 6) belongs to a group of Argonaute proteins and is the catalytic component of rna-containing silencing complex (RISC). This protein complex is responsible for gene silencing (RNAi).

For research use only, not for clinical use.