AGO5 | Argonaute 5
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AGO5 | Argonaute 5

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Cat: PA01335
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
peptide of Arabidopsis thaliana AGO5 UniProt: Q9SJK3, TAIR:AT2G27880
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunofluorescebce (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 100 (IF), 5 ug per gram floral tissue (IP), 1 : 1500 (WB)
Expected | apparent MW
111 | 111 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Arabidopsis thaliana
Not reactive in
Hordeum vulgare, Solanum lycopersicum, Zea mays

Additional information

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure

Description

AGO5 belongs to a group of argonaute proteins that are catalytic components of the RNA-including Silencing complex (RISC). This protein complex is responsible for gene silencing (RNAi). It may be related to antiviral RNA silencing.

For research use only, not for clinical use.