AGO4 | Argonaute 4

Inquiry

Cat: PA01334

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Hyacinthus orientalis

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5 , TAIR: At2g27040

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tube.

Application

CoImmunoprecipitation (IP) (coIP), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution

1 : 100 (ICC), 5 µg of antibody per 1 gram of a fresh tissue (IP),1 : 2000-1 : 5000 (WB)

Expected | apparent MW

103 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Hyacinthus orientalis

Predicted reactivity

Arabidopsis thaliana

Not reactive in

Brassica oleracea, Fragaria x ananassa, Hordeum vulgare, Solanum lycopersicum, Vigna angularis, Zea mays

Additional information

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa. Binds endogenous siRNAs, preference for 24nt siRNAs with 5' A.Note that the AGO4 antibody reacts with the NEB prestained protein marker.

Description

AGO4 (Argonaute 4) belongs to a group of Argonaute proteins and is the catalytic component of rna-containing silencing complex (RISC). This protein complex is responsible for gene silencing (RNAi).

For research use only, not for clinical use.