AGO2 | Argonaute 2

Inquiry

Cat: PA01333

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein, UniProt: Q9SHF3, TAIR: AT1G31280

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified IgY in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunoprecipitation (IP), Western blot (WB)

Recommended dilution

1 : 250-1 : 500 (WB)

Expected | apparent MW

113 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Capsella rubella, Solanum tuberosum

Not reactive in

Chlamydomonas reinhardtii, Medicago truncatula, Nicotiana benthamiana, Solanum lycopersicum, Zea mays

Additional information

AGO2 protein is strongly induced by stress. AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Antibody incubation should be done over night in 4°C. Use of material with enriched AGO2 levels is recommened.

Description

AGO2 belongs to a group of argonaute proteins that are catalytic components of the RNA-including Silencing complex (RISC). This protein complex is responsible for gene silencing (RNAi). AGO2 may be involved in antiviral RNA silencing.

For research use only, not for clinical use.