AGO1 | Argonaute 1

Inquiry

Cat: PA00772

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana

Datasheet:

Product Info

Immunogen

KLH-conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Immunolocalization (IL), small-RNA-IP-Seq, Western blot (WB)

Recommended dilution

2 µg (ChIP), 1: 200 (IF), (IL), small-RNA-IP-Seq, 1 : 5000-1 : 10 000 (WB)

Expected | apparent MW

116,4 | 130 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana

Predicted reactivity

Brassica pekinensis, Capsella rubella, Glycine max, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera

Not reactive in

Chlamydomonas reinhardtii, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea mays

Additional information

Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.To detect AGO1 in Nicotiana benthamiana, please inquire.Recommended for detection of AGO1: extreme low femtogram range chemiluminescent detection reagent,AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Buffer for extraction of AGO proteins: Paudel et al. 2018. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it is raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.TCA acetone precipitation method

Description

AGO1 belongs to a group of argonaute proteins that are catalytic components of the RNA-including Silencing complex (RISC). This protein complex is responsible for gene silencing (RNAi).

For research use only, not for clinical use.