ADH/ALDH | Alcohol/acetaldehyde dehydrogenase (bacterial/algal)

Inquiry

Cat: PA00489

Size: 200 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Chlamydomonas reinhardtii, E.coli, Streptococcus pmenumoniae

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from available algal and bacterial ADH sequences including Chlamydomonas reinhardtii

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

100 | 100 kDa (C.reinhardtii), 96 kDa (E.coli)

Reactivity

Confirmed reactivity

Chlamydomonas reinhardtii, E.coli, Streptococcus pmenumoniae

Predicted reactivity

Algae, Nannochloropsis gaditana, Picochlorum sp.

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Selected peptide is well conserved in Escherichia coli ADHE (P0A9Q7), most of the microbial dual function aldehyde/alcohol dehydrogenases (ADHE) and Iron-containing alcohol dehydrogenases are also conserved in a peptide used to elicit ADH antibody

Description

Alcohol dehydrogenase (e.c.:1.1.1.1) is an important enzyme in plants and microorganisms. In microalgae and bacteria, acetyl-CoA is catalyzed to ethanol by the bifolase alcohol/acetaldehyde dehydrogenase (ADH/ALDH) under hypoxia. Enamelled cable: 1.1.1.1/1.2.1.10). This reaction causes NAD+ circulation and allows glycolysis to take place.

For research use only, not for clinical use.