ADGP | ADP-glucose pyrophosphorylase

Inquiry

Cat: PA00488

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Horderum vulgare, Nicotiana tabacum, Triticum aestivum, Zea mays

Datasheet:

Product Info

Immunogen

part of Arabidopsis thaliana recombinant ADP-glucose pyrophosphorylase small subunit, TAIR: At5g48300, UniProt: P55228

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000-1 : 5000 (WB)

Expected | apparent MW

49,4 | 52 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Horderum vulgare, Nicotiana tabacum, Triticum aestivum, Zea mays

Predicted reactivity

Chlamydiae, Chlamydomonas reinhardtii, Cyanobacteria, Beta vulgaris, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Planctomycetes, Proteobacteria, Solanum lycopersicum, Spirochetes, Verrucomicrobia, Vitis vinifera

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

Cross reactivity of this antibody to Rubisco has been excluded using 2D gel electrophoresis.Antigen used to elicit this antbody has very poor conservation in large subunit of ADP-glucose pyrophosphorylase.

Description

Adp-glucose pyrophosphorylase (AGPase) catalyzes the first step in transient starch synthesis in plant chloroplasts and starch storage in amylosomes (teflow et al., 2004). AGPase in higher plants consists of two distinct subunits, encoded by different genes, forming the L2S2 heterotetramer. The large subunit (L) is a regulator of allosteric activity, while the small subunit (S) is a catalytic subunit (Kim et al., 2007). Synonyms: ADP-glucose pyrophosphorylase, ADP-glucose synthetase, AGPase B, α-D-glucose-1-adenosine phosphate transferase

For research use only, not for clinical use.