Adenylyltransferase and sulfurtransferase MOCS3 (MOCS3), Recombinant Protein

Full Product Name

Recombinant Oryza sativa subsp. japonica Adenylyltransferase and sulfurtransferase MOCS3 (MOCS3)

Product Gene Name

MOCS3 recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

MEGGGDDDGG RSRAEAIMRE LERLRAEREE LDGRIRLLES QLRLGAAPLP PSAAAEVEPT GSPSSSSSAA ADMISRYRRH LLLPQFGLEG QRKLSQSSIL VVGAGGLGSP VAMYLAACGV GCLGIVDGDR VELDNLHRQI IHIEAYVGQP KVKSTAASCR AYDIVVDATN NLPSRYMISD CCVLMNKPLI SGSAVGLEGQ LTVYHHNGSP CYRCLYPNPP SSPTSQSCSD NGILGILPGV IGCLQALEAI KVATAVGKPL CGRMLHFDAL SSHTRIVKIS RSSPTCKVCG ENPVFTKEDF VNFDYESFTQ SPMSKNSTTR SLNLLPENAR VSCRDYKKVL DSGRPHLLVD VRPSHHFQIA SMAHSINVPL SLLEEKLPLL RDSAREVSSR RDGRQHCPVY VICRRGNDSQ VAVQILRENG FLYASDVAGG FESWAKEVDP SFLLY

Sequence Positions

1-445, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

48,308 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI Accession #

A3ACF3.1

NCBI GI #

226707527

NCBI Official Full Name

Adenylyltransferase and sulfurtransferase MOCS3

UniProt Gene Name

MOCS3

UniProt Protein Name

Adenylyltransferase and sulfurtransferase MOCS3

UniProt Synonym Protein Names

Sulfur carrier protein MOCS2A sulfurtransferase

UniProt Primary Accession #

A3ACF3

UniProt Comments

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.