ACT | Actin (recombinant monoclonal, clone 14H4G8)
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ACT | Actin (recombinant monoclonal, clone 14H4G8)

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Cat: PA00995
Size: 50 µg
Host: Mouse
Clonality: Monoclonal
Confirmed reactivity: Arabidopsis thaliana, Medicago sativa, Zea mays
Datasheet:

Product Info

Immunogen
ca. 100 amino acids of recombinant actin conserved more than 80 %  in Arabidopsis thaliana: actin-1 P0CJ46  AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293  AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520
Host
Mouse
Clonality
Monoclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile or deionized water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1: 500 (IF), 1 : 1000 1: 5000 (WB)
Expected | apparent MW
41,6 | 45 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Medicago sativa, Zea mays
Predicted reactivity
Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao,Triticum aestivum,  Zea mays, Vicia faba
Not reactive in
Chlamydomonas reinhardtii

Additional information

This antibody is compatible with a secondary antibody for fluorescence detection:  goat anti-mouse secondary antibody (IRDye 800CW, Li-cor)

Description

Actin, a highly conserved protein, is an important part of the cytoskeleton and plays an important role in cytoplasmic flow, cell shape determination, cell division, organelle movement and extension growth. It is preferentially expressed in buds and expanding tissues, flower organ primordium, developing seeds and forming inflorescences.

For research use only, not for clinical use.