ACO1 | Aconitase

Arabidopsis expresses three highly similar aconitase isozymes (ACO1/ AT4G35830, ACO2/AT4G26970 and ACO3/AT2G05710), of which ACO1 is the cytosolic isoform, while ACO2 and ACO3 are predominantly located in the mitochondria (Arnaud et al 2007, Bernard et al 2009), The combined abundance and activity of the mitochondrial aconitases is about 3 times higher than the cytosolic pool (Bernard et al 2009),The Arabidopsis isoforms are more similar in amino acid sequence to mammalian iron-regulatory proteins (IRP-1) than to the mammalian and yeast mitochondrial aconitases,The antibody recognises all three Arabidopsis aconitase isoforms (ACO1, ACO2 and ACO3, see Bernard et al 2009), Possible differences in affinity have not been precisely quantified, Sensitivity threshold is between 2 and 10 ng for WB / ECL (see figure), Antibodies will recognize aconitase isoforms in denaturing and native gel electrophoresis

Aconitase is a single subunit enzyme of the tricarboxylic acid cycle (or Krebs cycle) in the mitochondria. Cytoplasmic isomers are also part of the glyoxylic acid cycle. Aconitase catalyzes the synthesis of isocitrate from citrate dehydration/water by cis-aconitase as an intermediate. Iron-sulfur clusters at the active site of the enzyme promoted the reaction. Iron-sulfur clusters are somewhat unstable, especially under oxidative stress, where the loss of cofactors leads to protein degradation. Alias :ACO, Citric acid hydrolase 1, 2, 3