ABI2 | Abscisic acid insensitive 2

Inquiry

Cat: PA01431

Size: 50 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide derived from N-terminus of Arabidopsis thaliana ABI2 sequence, UniProt: O04719, ,TAIR: AT5G57050 chosen peptide is not conserved in ABI1

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Expected | apparent MW

46 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

ABI2 antibodies recognize recombiant StrepTag-ABI2, ABI2-GST, His-ABI2. ABI2 protein is easily degraded therefore extraction buffer needs to contain protease inhibotors, example of such inhibitor coctail can be found here.To detect endogenous ABI2 plant material needs to be subjected to stress before harvesting.

Description

Abscisic acid-insensitive 2 (Protein Abscisic acid-insensitive 2, ABI2) is an inhibitor of the ABA signaling pathway, regulating various ABA reactions. Such as stomatal closure, plasma membrane permeability, strong light stress, glucose response, seed germination and vegetative growth inhibition. Related to acquired heat resistance. Alias: ABA-insensitive 2,ABI2, ATABI2, protein phosphatase 2C77, AtPP2C77, At5g57050, MHM17_19, MHM17.19, PP2C ABI2.

For research use only, not for clinical use.