A12,2 | RNA polymerase I subunit (homolog of Pol II Rpb9)
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A12,2 | RNA polymerase I subunit (homolog of Pol II Rpb9)

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Cat: PA01343
Size: 200 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence. This sequence is only weakly conserved in other eukaryotic sequences available in the databases.
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 143 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
13,6 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

This product has previously been labelled as anti-At3g25940 transcription factor S-II (TFIIS) domain-containing protein. Protocol for isolation of cytosolic and nuclear fractions can be found here.,This antibody is specific for A12,2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana

Description

RNA polymerase I is a DNA-dependent enzyme located in the nucleus that is involved in RNA elongation and transcriptional regulation. In yeast, subunit A12.2 is described as homologous to the Rpb9 subunit of polymerase II.

For research use only, not for clinical use.