95 kDa Lcm (phycobilisome - to- thylakoid core linker with phycocyanobilin chromophore)
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95 kDa Lcm (phycobilisome - to- thylakoid core linker with phycocyanobilin chromophore)

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Cat: PA01739
Size: 200 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Anabaena variablis, Aphanathece halophytica, Fremyella diplosiphon (Microchaete diplosiphon CCALA 811), Mastigocladus laminosus, Nostoc sp., Synechococcus sp. PCC 7002, ATCC 27264, PR6) , Synechococcus elongatus (strain PCC 7942), Anacystis nidulans R2), Synechococcus leopolensis (..elangatus PCC 6301), Synechecoccus lividus, Spirulina platensis (Athrospira platensis), Tolypothrix tenuis; red alga: Porphyridium cruentum (purpureum), P. aeruginuso, Rhodosorus marinus, Griffithsia monilis
Datasheet:

Product Info

Immunogen
native protein purified from phycobilisomes of Porphyridium cruentum
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 200 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 300-1 : 1000 (WB)
Expected | apparent MW
95 kDa (Porphyridium cruentum), 75-120 kDa (other species)

Reactivity

Confirmed reactivity
Anabaena variablis, Aphanathece halophytica, Fremyella diplosiphon (Microchaete diplosiphon CCALA 811), Mastigocladus laminosus, Nostoc sp., Synechococcus sp. PCC 7002, ATCC 27264, PR6) , Synechococcus elongatus (strain PCC 7942), Anacystis nidulans R2), Synechococcus leopolensis (..elangatus PCC 6301), Synechecoccus lividus, Spirulina platensis (Athrospira platensis), Tolypothrix tenuis; red alga: Porphyridium cruentum (purpureum), P. aeruginuso, Rhodosorus marinus, Griffithsia monilis
Predicted reactivity
Algae (red), Cyanobacteria
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Anabaena sp. sample can serve as a negative control, as under nitrogen deficient conditions phycobiliproteins are going to be lost. Overall sample quality is of crucial importance and in older or not properly stored samples, phycobiliproteins will undergo proteolytic degradation.

Description

A large polypeptide Lcm containing a small amount of phycocyanin chromophore that acts as a terminal energy receptor and a linking polypeptide (PS2) between phycocyanin bodies and photosynthetic reaction centers.

For research use only, not for clinical use.