5MeC | 5-Methylcytosine (clone 5MC-CD)

Inquiry

Cat: PA01299

Size: 100 µg

Host: Mouse

Clonality: Monoclonal

Confirmed reactivity: Chlamydmonas me-1 cells, mouse embryonic stem cells

Datasheet:

Product Info

Immunogen

BSA-conjugated 5-Methylcytosine

Host

Mouse

Clonality

Monoclonal

Purity

Purified IgM in PBS. Contains 50 % glycerol, filter sterilized.

Format

Liquid

Storage

Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunofluorescence (IF), Western blot (WB)

Recommended dilution

1:50 - 1: 100 (IF), 1 : 1000 (WB)

Reactivity

Confirmed reactivity

Chlamydmonas me-1 cells, mouse embryonic stem cells

Predicted reactivity

DNA with 5-Methylcytosine (methylated DNA)

Description

DNA methylation is a chemical modification of DNA that can be inherited and subsequently removed without altering the original DNA sequence. Therefore, it is part of the epigenetic code and is the most clearly characterized epigenetic mechanism. DNA methylation results in the addition of a methyl group to DNA - for example, on carbon 5 of the cytosine pyrimidine ring - which involves a reduction in gene expression. In adult somatic tissues, DNA methylation usually occurs in the context of CpG dinucleotides. Non-cpg methylation is prevalent in embryonic stem cells.

For research use only, not for clinical use.