5-hmC | 5-hydroxymethylcytosine (polyclonal)

Inquiry

Cat: PA01294

Size: 100 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Human

Datasheet:

Product Info

Immunogen

BSA-conjugated molecule: 5-hydroxymethylcytosine (5-hmC)

Host

Rabbit

Clonality

Polyclonal

Purity

Protein G purified.

Format

Liquid

Storage

Store lyophilized/reconstituted at -20°C; for long term storage Store at -80°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Dot blot (Dot), ELISA (ELISA), hMeDIP (hydroxymethylated DNA Immunoprecipitation (IP)

Recommended dilution

1 : 200 (Dot), 1 : 500 (ELISA), 2,5 µl/IP (hMeDIP)

Reactivity

Confirmed reactivity

Human

Predicted reactivity

Mouse, broad species range

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

This serum contains 0,05 % sodium azide

Description

5-methylol cytosine is a recently discovered DNA modification resulting from the conversion of 5-methylcytosine to 5-methylol cytosine by the TET oxygenase family. It may have an important role different from 5-methylcytosine (5-mC). The 5-hmC bases have been found in Purkinje neurons, granulosa cells, and embryonic stem cells, and they are present in Purkinje cells at high levels (up to 0.6% of the total nucleotides in Purkinje cells).

For research use only, not for clinical use.