4-coumarate--CoA ligase 1 (4CL1), Recombinant Protein
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4-coumarate--CoA ligase 1 (4CL1), Recombinant Protein

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Cat: RP01021
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Yeast)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana 4-coumarate--CoA ligase 1 (4CL1) , partial
Product Gene Name
4CL1 recombinant protein
Product Synonym Gene Name
4CL1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MAPQEQAVSQ VMEKQSNNNN SDVIFRSKLP DIYIPNHLSL HDYIFQNISE FATKPCLING PTGHVYTYSD VHVISRQIAA NFHKLGVNQN DVVMLLLPNC PEFVLSFLAA SFRGATATAA NPFFTPAEIA KQAKASNTKL IITEARYVDK IKPLQNDDGV VIVCIDDNES VPIPEGCLRF TELTQSTTEA SEVIDSVEIS PDDVVALPYS SGTTGLPKGV MLTHKGLVTS VAQQVDGENP NLYFHSDDVI LCVLPMFHIY ALNSIMLCGL RVGAAILIMP KFEINLLLEL IQRCKVTVAP MVPPIVLAIA KSSETEKYDL SSIRVVKSGA APLGKELEDA VNAKFPNAKL GQGYGMTEAG PVLAMSLGFA KEPFPVKSGA CGTVVRNAEM KIVDPDTGDS LSRNQPGEIC IRGHQIMKGY LNNPAATAET IDKDGWLHTG DIGLIDDDDE LFIVDRLKEL IKYKGFQVAP AELEALLIGH PDITDVAVVA MKEEAAGEVP VAFVVKSKDS ELSEDDVKQF VSKQVVFYKR INKVFFTESI PKAPSGKILR KDLRAKLANG L
Sequence Positions
1-561aa; Full Length
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
53,115 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

NCBI/Uniprot Data

NCBI Accession #
NP_175579.1
NCBI GI #
15218002
NCBI GenBank Nucleotide #
NM_104046.3
NCBI GeneID
841593
NCBI Official Full Name
4-coumarate:CoA ligase 1
NCBI Official Symbol
4CL1
NCBI Official Synonym Symbols
4-COUMARATE:COA LIGASE; 4-coumarate:CoA ligase 1; 4-COUMARATE:COA LIGASE 1; 4CL.1; ARABIDOPSIS THALIANA 4-COUMARATE:COA LIGASE 1; AT4CL1; F19C24.11; F19C24_11
NCBI Protein Information
4-coumarate:CoA ligase 1
NCBI Summary
encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. In addition to 4-coumarate, it also converts ferulate. The catalytic efficiency was in the following (descending) order: p-coumaric acid, ferulic acid, caffeic acid and 5-OH-ferulic acid. At4CL1 was unable to use sinapic acid as substrate.
UniProt Gene Name
4CL1
UniProt Synonym Gene Names
4CL 1; At4CL1
UniProt Protein Name
4-coumarate--CoA ligase 1
UniProt Synonym Protein Names
4-coumarate--CoA ligase isoform 1; At4CL1; 4-coumaroyl-CoA synthase 1
UniProt Primary Accession #
Q42524
UniProt Secondary Accession #
Q8RY63
UniProt Related Accession #
Q42524
UniProt Comments
Produces CoA thioesters of a variety of hydroxy- and methoxy-substituted cinnamic acids, which are used to synthesize several phenylpropanoid-derived compounds, including anthocyanins, flavonoids, isoflavonoids, coumarins, lignin, suberin and wall-bound phenolics.

For research use only, not for clinical use.